Journal: Cell reports

Article Title: A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions

doi: 10.1016/j.celrep.2025.115346

Figure Lengend Snippet: (A) Schematic of experimental approach. Antisense PMOs are delivered into cells. PMOs targeting the GA-rich region of the RAB13 mRNA prevent RAB13 localization, potentially through interfering with RBP binding. (B and C) CNBP (B) or KIF1C (C) immunoprecipitation to detect amount of associated RNAs after PMO delivery. Note that binding to RAB13 mRNA is specifically affected upon delivery of PMOs targeting the RAB13 GA-region. n = 5 (B) and 7 (C) independent replicates. Error bars: SEM. *** p < 0.001, ** p < 0.01, and ns, non-significant, by Kruskal-Wallis test with Dunn’s multiple comparisons test. (D and E) Relative amount of CNBP (D) or KIF1C (E) protein recovered in immunoprecipitates (IPs) by western blot. ns, non-significant, by Wilcoxon matched-pairs signed-rank test. (F) Proposed model for motor-adaptor complex directing mRNA trafficking to cell protrusions. CNBP binds to GA-rich regions within the 3′ UTRs of protrusion-targeted mRNAs and serves as an adaptor for the recruitment of the KIF1C kinesin. Additional factors could participate in this complex. KIF1C subsequently traffics mRNAs on microtubules toward the cell periphery.

Article Snippet: In several cases, localization to protrusions relies on GA-rich regions within the 3′ UTRs of targeted mRNAs., , , In the case of the human RAB13 mRNA, when a specific ~50 nt GA-rich region within the 3′ UTR is deleted, or when its function is interfered with using antisense oligonucleotides (oligos), peripheral RAB13 mRNA localization is impaired.

Techniques: Binding Assay, Immunoprecipitation, Western Blot